faircloth-lab illumina library prep protocol¶
Release v2.1. (Changelog)
|Author:||Brant C. Faircloth, Travis C. Glenn, Noor D. White|
|Date:||23 February 2014 20:17 CST (-0600)|
|Copyright:||This documentation is available under a Creative Commons (CC-BY) license.|
Suggested Reading / References¶
|[Blum2010]||Blumenstiel B et al. 2010. Targeted exon sequencing by in-solution hybrid selection. Curr Protoc Hum Genet Chapter 18:Unit 18.4.|
|[Fair2012]||Faircloth BC, Glenn TC: Not all sequence tags are created equal: designing and validating sequence identification tags robust to indels. PLoS One 7: e42543.|
|[Fish2011]||Fisher S, et al. 2011. A scalable, fully automated process for construction of sequence-ready human exome targeted capture libraries. Genome Biol 12:R1.|
|[Gnir2009]||Gnirke A et al. 2009. Solution hybrid selection with ultra-long oligonucleotides for massively parallel targeted sequencing. Nature Biotechnology 2009, 27:182–189.|
|[Kapa2011]||Kapa Biosystems. Kapa Library Preparation Kits, Illumina Series, version 2.11. 2011. Cape Town, South Africa.|
|[NEB2011]||New England Biolabs. NEBNext DNA Sample Prep Master Mix Set 1, Illumina Compatible, version 2.0. 2011. Ipswich, MA, USA.|
Prepare DNA libraries having unique sequence tagged adapters ligated to DNA fragments on a per-library basis.
This protocol incorporates the with-bead AMPure cleanup protocol, initially described in [Fish2011], which conducts all of the library preparation steps in the presence of SPRI/AMPure/SeraPure beads.